Concentration Assay:Article Title: Emerin is an effector of oncogenic KRAS-driven nuclear dynamics in pancreatic cancer
Article Snippet: .. Target Company Catalog # RRID# Concentration Western Blot Emerin Abcam ab156871 1:5000 Emerin Proteintech 0351-1-AP AB_2100056 1:5000 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:2500 LAP2b Proteintech 14651-1-AP AB_2205440 1:1000 Lamin B1 Abcam ab16048 AB_443298 1:7000 Lamin B2 Abcam ab151735 AB_2827514 1:7000 Sun1 Aviva Systems Biology ARP49929_P050 AB_1295001 1:1000 Sun2 Proteintech 27556-1-AP AB_2880906 1:4000 Anti-HA Roche 11867423001 AB_390918 1:1000 p44/42 (Erk 1/2) Cell Signaling 9107S 1:1000 p44/42 MAPK (Erk1/2) (137F5) Cell Signaling 4695S 1:1000 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1000 Vinculin Bethyl A302-535A AB_1999080 1:10,000 Goat Anti-Rabbit IgG EMD AP132P AB_90264 1:10,000 Goat Anti-Mouse IgG EMD AP124P AB_90456 1:10,000 Goat Anti-Rat IgG EMD AP136P AB_11214444 1:10,000 Immunofluorescence Emerin Abcam ab156871 1:150 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:400 Lamin B1 Abcam ab16048 AB_443298 1:250 Anti-HA Cell Signaling 2367S 1:100 Insulin Abcam ab7842 1:100 Glucagon Sigma-Aldrich G2654 1:1000 α-Amylase Sigma-Aldrich A8273 1:100 Goat anti-Rabbit Alexa Fluor 594 Thermo Fisher Scientific A-11037 AB_2534095 1:200 Goat anti-Rabbit Alexa Fluor 488 Molecular Probes A-11008 AB_143165 1:200 Goat anti-Rabbit Alexa Fluor 568 Thermo Fisher Scientific A-11011 AB_143157 1:200 Goat anti-Mouse Alexa Fluor 488 Thermo Fisher Scientific A-11001 AB_2534069 1:200 Goat anti-Mouse Alexa Fluor 594 Thermo Fisher Scientific A-11005 AB_2534073 1:200 Goat anti-Chicken Alexa Fluor 647 Thermo Fisher Scientific A-21449 AB_2535866 1:200 Immunohistochemistry p44/42 MAPK (Erk1/2) (3A7) Cell Signaling 9107S 1:1200 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1200 ..
Western Blot:Article Title: Emerin is an effector of oncogenic KRAS-driven nuclear dynamics in pancreatic cancer
Article Snippet: .. Target Company Catalog # RRID# Concentration Western Blot Emerin Abcam ab156871 1:5000 Emerin Proteintech 0351-1-AP AB_2100056 1:5000 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:2500 LAP2b Proteintech 14651-1-AP AB_2205440 1:1000 Lamin B1 Abcam ab16048 AB_443298 1:7000 Lamin B2 Abcam ab151735 AB_2827514 1:7000 Sun1 Aviva Systems Biology ARP49929_P050 AB_1295001 1:1000 Sun2 Proteintech 27556-1-AP AB_2880906 1:4000 Anti-HA Roche 11867423001 AB_390918 1:1000 p44/42 (Erk 1/2) Cell Signaling 9107S 1:1000 p44/42 MAPK (Erk1/2) (137F5) Cell Signaling 4695S 1:1000 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1000 Vinculin Bethyl A302-535A AB_1999080 1:10,000 Goat Anti-Rabbit IgG EMD AP132P AB_90264 1:10,000 Goat Anti-Mouse IgG EMD AP124P AB_90456 1:10,000 Goat Anti-Rat IgG EMD AP136P AB_11214444 1:10,000 Immunofluorescence Emerin Abcam ab156871 1:150 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:400 Lamin B1 Abcam ab16048 AB_443298 1:250 Anti-HA Cell Signaling 2367S 1:100 Insulin Abcam ab7842 1:100 Glucagon Sigma-Aldrich G2654 1:1000 α-Amylase Sigma-Aldrich A8273 1:100 Goat anti-Rabbit Alexa Fluor 594 Thermo Fisher Scientific A-11037 AB_2534095 1:200 Goat anti-Rabbit Alexa Fluor 488 Molecular Probes A-11008 AB_143165 1:200 Goat anti-Rabbit Alexa Fluor 568 Thermo Fisher Scientific A-11011 AB_143157 1:200 Goat anti-Mouse Alexa Fluor 488 Thermo Fisher Scientific A-11001 AB_2534069 1:200 Goat anti-Mouse Alexa Fluor 594 Thermo Fisher Scientific A-11005 AB_2534073 1:200 Goat anti-Chicken Alexa Fluor 647 Thermo Fisher Scientific A-21449 AB_2535866 1:200 Immunohistochemistry p44/42 MAPK (Erk1/2) (3A7) Cell Signaling 9107S 1:1200 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1200 ..
Immunofluorescence:Article Title: Emerin is an effector of oncogenic KRAS-driven nuclear dynamics in pancreatic cancer
Article Snippet: .. Target Company Catalog # RRID# Concentration Western Blot Emerin Abcam ab156871 1:5000 Emerin Proteintech 0351-1-AP AB_2100056 1:5000 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:2500 LAP2b Proteintech 14651-1-AP AB_2205440 1:1000 Lamin B1 Abcam ab16048 AB_443298 1:7000 Lamin B2 Abcam ab151735 AB_2827514 1:7000 Sun1 Aviva Systems Biology ARP49929_P050 AB_1295001 1:1000 Sun2 Proteintech 27556-1-AP AB_2880906 1:4000 Anti-HA Roche 11867423001 AB_390918 1:1000 p44/42 (Erk 1/2) Cell Signaling 9107S 1:1000 p44/42 MAPK (Erk1/2) (137F5) Cell Signaling 4695S 1:1000 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1000 Vinculin Bethyl A302-535A AB_1999080 1:10,000 Goat Anti-Rabbit IgG EMD AP132P AB_90264 1:10,000 Goat Anti-Mouse IgG EMD AP124P AB_90456 1:10,000 Goat Anti-Rat IgG EMD AP136P AB_11214444 1:10,000 Immunofluorescence Emerin Abcam ab156871 1:150 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:400 Lamin B1 Abcam ab16048 AB_443298 1:250 Anti-HA Cell Signaling 2367S 1:100 Insulin Abcam ab7842 1:100 Glucagon Sigma-Aldrich G2654 1:1000 α-Amylase Sigma-Aldrich A8273 1:100 Goat anti-Rabbit Alexa Fluor 594 Thermo Fisher Scientific A-11037 AB_2534095 1:200 Goat anti-Rabbit Alexa Fluor 488 Molecular Probes A-11008 AB_143165 1:200 Goat anti-Rabbit Alexa Fluor 568 Thermo Fisher Scientific A-11011 AB_143157 1:200 Goat anti-Mouse Alexa Fluor 488 Thermo Fisher Scientific A-11001 AB_2534069 1:200 Goat anti-Mouse Alexa Fluor 594 Thermo Fisher Scientific A-11005 AB_2534073 1:200 Goat anti-Chicken Alexa Fluor 647 Thermo Fisher Scientific A-21449 AB_2535866 1:200 Immunohistochemistry p44/42 MAPK (Erk1/2) (3A7) Cell Signaling 9107S 1:1200 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1200 ..
Immunohistochemistry:Article Title: Emerin is an effector of oncogenic KRAS-driven nuclear dynamics in pancreatic cancer
Article Snippet: .. Target Company Catalog # RRID# Concentration Western Blot Emerin Abcam ab156871 1:5000 Emerin Proteintech 0351-1-AP AB_2100056 1:5000 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:2500 LAP2b Proteintech 14651-1-AP AB_2205440 1:1000 Lamin B1 Abcam ab16048 AB_443298 1:7000 Lamin B2 Abcam ab151735 AB_2827514 1:7000 Sun1 Aviva Systems Biology ARP49929_P050 AB_1295001 1:1000 Sun2 Proteintech 27556-1-AP AB_2880906 1:4000 Anti-HA Roche 11867423001 AB_390918 1:1000 p44/42 (Erk 1/2) Cell Signaling 9107S 1:1000 p44/42 MAPK (Erk1/2) (137F5) Cell Signaling 4695S 1:1000 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1000 Vinculin Bethyl A302-535A AB_1999080 1:10,000 Goat Anti-Rabbit IgG EMD AP132P AB_90264 1:10,000 Goat Anti-Mouse IgG EMD AP124P AB_90456 1:10,000 Goat Anti-Rat IgG EMD AP136P AB_11214444 1:10,000 Immunofluorescence Emerin Abcam ab156871 1:150 Lamin A+ Lamin C Abcam ab8984 AB_306913 1:400 Lamin B1 Abcam ab16048 AB_443298 1:250 Anti-HA Cell Signaling 2367S 1:100 Insulin Abcam ab7842 1:100 Glucagon Sigma-Aldrich G2654 1:1000 α-Amylase Sigma-Aldrich A8273 1:100 Goat anti-Rabbit Alexa Fluor 594 Thermo Fisher Scientific A-11037 AB_2534095 1:200 Goat anti-Rabbit Alexa Fluor 488 Molecular Probes A-11008 AB_143165 1:200 Goat anti-Rabbit Alexa Fluor 568 Thermo Fisher Scientific A-11011 AB_143157 1:200 Goat anti-Mouse Alexa Fluor 488 Thermo Fisher Scientific A-11001 AB_2534069 1:200 Goat anti-Mouse Alexa Fluor 594 Thermo Fisher Scientific A-11005 AB_2534073 1:200 Goat anti-Chicken Alexa Fluor 647 Thermo Fisher Scientific A-21449 AB_2535866 1:200 Immunohistochemistry p44/42 MAPK (Erk1/2) (3A7) Cell Signaling 9107S 1:1200 p-Erk/P-p44/42 MAPK (T202/Y204) (D13.14.4E) XP Cell Signaling 4370S 1:1200 ..
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